Solid phase radioimmunoassay of digoxin

Hersh, Leroy S.; Yaverbaum, Sidney;

Clinically significant concentrations of digoxin can be determined by reacting an unknown amount of digoxin, a known amount of labelled digoxin, and a composite consisting of anti-digoxin antibodies coupled through an intermediate silane to magnetically responsive particles; magnetically separating the immunochemical complexes formed thereby, counting the radioactivity of either the separated products or remaining solution, and relating the count to a standard curve.






BACKGROUND OF THE INVENTION

1. Field of the Invention

This invention relates generally to radioimmunoassay and specifically to solid phase radioimmunoassay of digoxin.

Radioimmunoassay (RIA) is a term used to describe any of several methods for determining very low concentrations of substances, which methods are based on the use of radioactively labelled substances which can form immunochemical complexes. The RIA of a substance for which there exists antibodies is based on the observation that an unknown amount of that substance will tend to compete equally with a known amount of that substance (radioactively labelled) for a limited number of complexing sites on antibodies to the substance. Thus, by permitting the above reaction, and separating the immunochemical complexes formed thereby from the reaction solution, the unknown concentration can be determined by relating the radioactivity count of the separated products or remaining solution to a standard curve prepared beforehand using known amounts of labelled and unlabelled substances.

An essential step in RIA is the separation of the immunochemical complexes from the reaction solution. To facilitate this step, various techniques have been devised to immobilize anti-substance antibodies on essentially water-insoluble carrier materials so that a relatively rapid separation can be accomplished with readily available equipment (e.g. a centrifuge). Methods for immobilizing antibodies on such carriers can be found in U.S. Pat. No. 3,555,143, (organic carriers) issued to Axen et al. on Jan. 12, 1971 and U.S. Pat. No. 3,652,761, (inorganic carriers) issued to H. H. Weetall on Mar. 28, 1972. When such carrier materials are used as supports for antibodies in RIA, it has become a common practice to refer to the technique as solid-phase RIA or, simply, SPRIA. The present invention discloses a novel SPRIA for digoxin.

Digoxin is a cardiac glycoside commonly used in very small quantities as a heart stimulant. The difference between therapeutic and toxic amounts of digoxin is often very slight. Thus, it is very important to have methods for accurately determining very small concentrations of digoxin in serum or plasma samples. Since the clinically significant concentration range of digoxin is within the broad range of about 0.5ng to about 10 ng per ml, RIA offers the only practical method for determining digoxin concentrations.

2. Description of Prior Art

Although conventional RIA techniques are known for measuring digoxin concentrations, those techniques are often time-consuming because they require relatively long periods of time for complete separation of digoxin-anti-digoxin antibody complexes. Hence, because of the importance knowing digoxin concentrations as rapidly as possible, attention has been made in recent years to develop a SPRIA for digoxin which would permit rapid separation and accuracy within the clinically significant concentration range. Methods for conjugating digoxin residues to the amino groups of lysine residues in human serum albumin are disclosed by T. W. Smith et al. in Biochemistry, 9, No. 2, 331-337 (1970) and by V. P. Bulter et al. in Proc. N.A.S., 57, 71-78 (1966). A method of labelling a digoxin derivative with .sup.125 I is disclosed by Gutcho et al. in Clin. Chem. 19/9, 1058-59 (1973). As mentioned above, various techniques for coupling antibodies to inorganics through silanes are disclosed in U.S. Pat. No. 3,652,761, issued to H. H. Weetall. In those disclosures which disclose a specific SPRIA for digoxin, however, the methods of separating immunochemical complexes generally involve using a centrifuge. Although the use of a centrifuge facilitates separation and hastens obtaining the assay results, the use of a centrifuge does not readily permit instrumentation of the SPRIA of digoxin. Hence, there has been a need for another method of separating the reaction products of SPRIA which method could be readily instrumentized for quicker assay results.

In an article by P. J. Robinson et al in Biotech. Bioeng. XV, 603-606 (1973) there are suggested various techniques for separating immobilized enzymes by coupling the enzymes to magnetically responsive inorganic particles and then using magnetism to effect the separation. We are unaware, however, of the use of a similar technique to provide for magnetic separations in a SPRIA of digoxin, especially in a SPRIA of digoxin which can be successfully used to measure clinically significant amounts of digoxin. We have found that a successful SPRIA of digoxin is possible using magnetically responsive carrier materials. The SPRIA lends itself to instrumentation. Details of our composites and methods of making and using them are described below.

SUMMARY OF INVENTION

Our method for determining the concentration of digoxin, especially within the clinically significant concentration range of about 0.5 ng/ml to about 10 ng/ml., comprises the steps of:

A. reacting a solution containing an unknown amount of digoxin, a known amount of radioactively labelled digoxin, and composites consisting of antidigoxin antibodies coupled chemically through an intermediate silane coupling agent to magnetically responsive inorganic particles to form immunochemical complexes;

B. magnetically separating the composites from the reaction solution;

C. counting the radioactivity of either the removed composites or the remaining solution; and

D. relating the radioactivity count of step (C) to a standard to determine the digoxin concentration.

In a preferred embodiment, our method comprises using a composite consisting of anti-digoxin antibodies coupled through a silane coupling agent to Fe.sub.3 O.sub.4 particles having an average particle size between about 1.5 and 10 microns, and having a surface area of at least about 10 m.sup.2 /g,

BRIEF DESCRIPTION OF THE FIGURES

FIG. 1 is a graph illustrating the biological activity at various dilutions of magnetite-anti-digoxin antibody composites at ambient temperatures in the presence and absence of human plasma.

FIG. 2 is a graph illustrating a standard digoxin assay curve generated using one of our composites.

SPECIFIC EMBODIMENTS

A very important aspect of our SPRIA of digoxin is the carrier for the anti-digoxin antibodies. The carrier must be magnetically responsive; that is, responsive to magnetic forces such that a suspension of carrier particles can be removed from a solution with the aid of a magnet, magnets, or magnetic forces. Further, the carrier must be capable of being silanized so that the anti-digoxin antibodies (antisera) can be coupled chemically through a silane to the carrier. As used herein, the term silanized, or its equivalent, refers to an inorganic carrier which has attached to the surface thereof compounds known as silanes and which compounds are capable, alone, or through modification, of reacting with anti-digoxin antibodies to chemically couple the antibodies to the carrier without significant loss of antibody complexing ability.

As shown in U.S. Pat. No. 3,652,761, (coupling of antibodies and antigenic substances to silanized inorganics) and U.S. Pat. No. 3,519,538. (coupling enzymes to silanized inorganics) the inorganic carrier materials should have available surface oxide or hydroxyl groups with which silanes can react for attachment of the silane. Since our carrier must be both magnetically responsive and capable of being silanized, it is contemplated that our SPRIA of digoxin can use anti-digoxin carriers of the following materials: Fe.sub.3 O.sub.4, CoO, NiO, Mn.sub.2 O.sub.3, and finely divided iron and nickel particles which have oxide surfaces. Such materials can be readily silanized by known means. In view of the various coupling techniques disclosed in U.S. Pat. No. 3,652,761, and this disclosure, those skilled in the art will be able to devise numerous methods of coupling active anti-digoxin antibodies by appropriately modifying the attached silanes for coupling with the antibodies in such a manner that the complexing ability of the coupled antibody is not lost.

In addition to the requirements of being magnetically responsive and capable of being silanized, our carriers should be essentialy water-insoluble and have a relatively high surface area (e.g. greater than about 10 m.sup.2 /g) to assure sufficient loading of anti-digoxin antibodies. Sensitivity of our SPRIA, especially in the clinically significant concentration range of digoxin, requires that such a relatively large surface area be available for antibody loading. Further, since immunochemical complexing is enhanced by assuring maxium exposure of the loaded antibody to the reaction or incubation solution, our average carrier particle size is such that the carrier-antibody composites will remain in suspension in the reaction solution for a period of time sufficient to assure enough complexing that will permit an accurate count and concentration determination (e.g. the particles should be small enough to remain in suspension for at least about 5 minutes). To achieve the above requirements, our carrier consists of silanizable, magnetically responsive inorganic particles having an average particle size between about 1.5.mu. and about 10.mu., preferably between about 1.5.mu. and about 2.5.mu..

In the illustrative examples below, anti-digoxin (goat) antiserum obtained from Biospheres, Inc. of Miami, Fla. and having a titer of at least 1:50,000, was coupled chemically through a diazotized silane coupling agent to particles of Fe.sub.3 O.sub.4 as described in greater detail hereunder. The Fe.sub.3 O.sub.4 particles were obtained from Fischer Scientific Co. (I-119, lot no. 765853) and consisted of purified Fe.sub.3 O.sub.4 particles having a particle size distribution such that about 50% of the particles were equal to or slightly larger than 2.5.mu.. Substantially all the particles had a size between about 1.5 and 10 microns.

The particles were silanized by using an arylamine trimethoxy silane (Union Carbide Y-5475). The silanized carrier was then diazotized and coupled to the anti-digoxin antiserum, as indicated. Biological activity (or complexing ability) of the resulting composites were then determined and a typical standard curve (standard) was prepared as described, using .sup.3 H-Digoxin (tritiated digoxin).

Preparation of Arylamine-Magnetite

One ml of arylamine trimethoxy silane (Union Carbide Y-5475) was dissolved in 100 ml of CH.sub.3 OH. Then 10 ml of distilled water and 0.4 g of orthophosphorous acid [H.sub.2 (HPO.sub.3)] were added and the solution was stirred for 15 minutes. Finally, 2 gms of the above-described Fe.sub.3 O.sub.4 particles were added and the suspension was swirled for 2 hours. The solution was decanted and the silanized Fe.sub.3 O.sub.4 particles were blotted dry before vacuum drying at 90.degree.C for 24 hours. The silanized Fe.sub.3 O.sub.4 was then rinsed three times with CH.sub.3 OH and air dried.

Preparation of Magnetite-Digoxin Antiserum Composite

To 2g of arylamine-magnetite wet cake (ice-cold) were added with stirring, 10 ml of 2N HC1 and 0.25 g of solid NaNO.sub.2. The reaction mixture was placed in a vacuum desiccator for 40 minutes. The diazotized magnetite was washed and added in increments to 1.5 ml goat anti-digoxin serum, previously adjusted to pH 8.3 with 0.1 N NaOH. The coupling procedure was performed in an iced test tube, and the pH was maintained between 7.5 and 8.5 with 0.1 N NaOH. The reaction was continued for 2 hours in this manner. The pH was at 8.5 for the last 30 minutes. The reaction mixture was refrigerated overnight. The mixture was washed four times with borate buffered saline (BBS-0.01 M Borate, pH 8.5, in 0.15M-NaCl) and stored as a wet cake at 5.degree.C.

Analysis of the wet cake showed that it was 46.3% dry weight and contained 11.7 mg protein/g dry weight of magnetite (as determined by Ninhydrin nitrogen determination procedure).

A suspension of 1.97 mg of the magnetite-antiserum IMA (immobilized antiserum) wet cake was made in 4 ml of 0.01 M PBS-BSA buffer (0.01 M phosphate, pH 7.4, in 0.15 M NaCl containing 3.5 mg bovine serum albumin and 0.0002% NaN.sub.3). This was equivalent to 266.76 .mu.g antiserum protein and 22.8 mg of IMA (dry weight) per ml of buffer suspension. The above is referred to herein as the stock suspension.

The sedimentation rate was measured without the presence of a magnetic field. The absorbance at 500 nm was followed after adding a mixed suspension into a cuvette. The absorbance decreased to about 20% of the initial value in 14 minutes. The t.sub.1/2 was 5 minutes.

Determination of Biological Activity

An experimental protocol was prepared to test the IMA for biological activity. Portions of the above stock suspension were brought to ambient temperature and diluted 1:25, 1:50 and 1:100 in filtered distilled water (FDW). The protocol shown in Table 1 is a combination of two experiments.

The experiments were performed by placing all protocol reagents except the .sup.3 H-Digoxin into each of 5 tubes with stirring. All assay tubes were activated by the addition of .sup.3 H-Digoxin at one time. The reaction of one tube of each IMA dilution was terminated 7.5, 17.5 and 27.5 minutes after delta zero time (1.25 minutes). All tubes were stirred every 10 minutes. Termination of the reaction and separation of bound from free .sup.3 H-Digoxin was effected by holding the butt of the reaction test tube to a powerful magnet for 45 seconds. The magnet was an Alnico V magnet obtained from Edmund Scientific Co., said to be capable of lifting about 150 lbs. The supernatant solution was decanted into a scintillation vial containing 10 ml of Insta-Gel (Packard Corp.) while still holding the reaction tube butt to the magnet. The vials were counted for 1 minute in a Packard Tri-Carb Liquid Scintillation Spectrometer, Model 3320.

                                      TABLE I
    __________________________________________________________________________
    Experimental Protocol for the Determination of Biological Activity at
    Ambient Temperature
    of a Preparation of Digoxin Antiserum Immobilized on Magnetite
                          IMA Final Concentration
                Dilution of
                          Antiserum
                                Dry Weight   Human Plasma  .sup.3 H-Digoxin*
    Sample Content
                Stock Suspension
                          Protein mg
                                of IMA mg
                                       FDW, ml.
                                             ml.     IMA, ml.
                                                           ml.
    __________________________________________________________________________
    Radioactive Antigen
    Control:
          1.    --        --    --     0.8   --      --    0.2
          2..sup.#
                --        --    --     0.6   0.2     --    0.2
    Stock Suspension
                Undiluted 53.350
                                4.560  0.6   --      0.2   0.2
    Stock Suspension
                1:25.sup.#
                          2.130 0.1825 0.4   0.2     0.2   0.2
    Stock Suspension
                1:50      1.065 0.0913 0.6   --      0.2   0.2
    Stock Suspension
                1:100     0.534 0.0450 0.6   --      0.2   0.2
    __________________________________________________________________________
     *0.64 ng .sup.3 H-Digoxin and 5 nci/0.2 ml.
     .sup.#Experiment No. 2.


The results of the experiment are shown on FIG. 1 which illustrates the biological activity of the magnetite-digoxin antiserum at ambient temperature in the presence and absence of human plasma. In FIG. 1, A represents the undiluted (1:5 final) sample, B represents the 1:50 dilution (1:250 final), C represents the 1:25 (1:125 final) dilution in the presence of human plasma, and D represents the 1:100 (1:500 final) sample dilution. Good activity was demonstrated for all dilutions. However, because sensitivity and standard curve data would be collected in the presence of human plasma, the 1:25 (1:125 final dilution) suspension was selected for preparing a standard curve. The higher IMA dilutions would probably show lower binding capacities in the presence of human plasma (see FIG. 1) at 17.5 minutes reaction time. This would not be advantageous for the assay.

Standard Curve

A dilution of 1:25 of the stock solution was selected to prepare the standard curve in the presence of human plasma. The protocol for the standard curve was similar to the one shown in Table 1 (Experiment No. 2) with the addition that concentrations of unlabeled digoxin (0.5, 1.0, 2.0, 5.0 and 10.0 mg/ml) in human plasma were included. The digoxin standard tubes were prepared in duplicate by adding 0.2 ml of filtered normal plasma containing known concentrations of digoxin to 0.4 ml of filtered distilled water, followed by 0.2 ml of the 1:25 dilution of IMA. Controls contained only the filtered distilled water and normal human plasma. Mixtures were stirred with each addition. Tubes were activated at 1 minute intervals by the addition of 0.2 ml of .sup.3 H-Digoxin (containing 0.64 ng digoxin and 5 nci activity as soon after the other reagents had been added. With the addition of .sup.3 H-Digoxin, each tube contained a 1.0 ml total volume. All tubes were stirred at 5 minute intervals. Reaction time was 17.5 minutes. Termination of the reaction and separation of the bound and free phases was effected by sedimentation and decantation in a magnetic field with the large magnet. The supernatant liquid was mixed with 10 ml of Insta-Gel in a scintillation vial and counted on the Packard Model 3320 for 1 minute.

Results of the experiments are summarized in Table 2, and graphically demonstrated in FIG. 2. The data show a .DELTA.% binding of 33% and a slope of 0.845. The immobilized antiserum preparation had 47% of the native antibody activity of the soluble antiserum (1:70,000 dilution of goat anti-digoxin serum, 70 mg protein/ml) on a protein weight basis.

                  TABLE 2
    ______________________________________
    Standard Curve # Data for Digoxin
    Antiserum - Magnetite Composite.*
                              % Free   % Bound
    Sample           cpm      Digoxin  Digoxin
    ______________________________________
    3H-Digoxin Control
                     3990     100.0    0.0
    O ng  Digoxin/ml Plasma
                         2514     63.0   37.0
    0.5   "              2579     64.7   35.3
    1.0   "              2725     68.3   31.7
    2.0   "              3002     75.2   24.8
    5.0   "              3390     85.0   15.0
    10.0  "              3836     96.2   3.8
    ______________________________________
     #Ambient temperature, 17.5 minutes reaction time.
     *2.13 .mu.g Antiserum protein/182.5 .mu.g of complex.


The above experiments indicate that clinically significant concentrations of digoxin can be determined quickly and accurately using our magnetically separable antibody-carrier-composite. A workable standard curve was obtained in the range of 1 to 10 ng digoxin per ml of plasma. The protein loading was a respectable 1% (dry weight) for a nonporous body having a high surface area. Inasmuch as our illustrative methods and composites can be readily modified given the disclosure herein, it is intended that the above examples should be construed as merely illustrative and the scope of the present invention be limited only by the appended claims.

Diazotype multicolor reproduction process
Cable tension roller
Automatic pistol
Cardiac and vascular prostheses
Electro-acoustic transducer
Antimicrobial composition
Multifunctional reference electrode
Underwater communications system
Klebsiella pneumoniae and Enterobacter broth
Snap action switches
Portable wine dispenser
Current scaling circuits
Electrical connection for electrodes
Packing for technical processes
Method for removing moisture particles
Failsafe logic function apparatus
Production of mushroom spawn
Automatic train stopping
Method of making non-woven fabrics
Dye lasers
Polishing wheels
Microwave device
Powdered carpet composition
Laminated pier bumper
Shear-stabilized emulsion flooding process
Multipassage diffuser
Pneumatically operated gated irrigation system
Automobile frame alignment apparatus
Fastener strip
High speed lubricated bearing
Copy paper feeding cassette
Catalytic cracking
Electrical connector
Fish lure desnagger
Method of combining synthetic yarns
Drive line safety shield
Monensin urethane derivatives
Dual wheel adapter kit
Method for master pattern production
Cigarette holder for ash receptacles
Robot movable in a group
Connector with improved terminal support
Scan interlock system
Recessed lighting fixture
9-Deoxy-9-methylene-PGF-3,4-didehydropiperidylamides
Manifold assembly fastening
Self-retaining electrical terminal
Building deck construction
Prostaglandin intermediates
Rotary cocks carrying spherical seals
Golf glove
High frequency ventillator
Copying objective lens system
Generating pulses
Cargo roller
Gathering implement
Safety bindings for skis
Timing device for fluid valve
Device for inhaling powdered substance
Panel assembling method
Synchronous transmission control system
Safety sleeve
Trifluoromethyl-substituted phenyl acetonitriles
Lubricant compositions
Self-timing automatic conveyor system
Apparatus for totalizing sales
Herbicidal sulfonamides
Pacemaker training aid
Polymer emulsification process
Nitrophenyl imino propionates
Pocket button attaching device
Glass units
Expendable case ammunition
Information transmission system
Multi-copy ion-valve radiography
Upholstered article and method
Ball handling article
Toe iron
3-Triazolylthio derivatives of ureido cephalosporins
Marine engine noise suppressor
Engine emission pollutant separator
Method for continuous extrusion
Method of treating bacterial infections
Packaged electric lamp
Automatic bowling lane stripper
Power transmission for an automobile
Vehicle speed control apparatus
Pyrolysis apparatus
Internal combustion engine with supercharger
Electronically controllable filter
Power breaker system
Lincomycin 3-(5'-adenylate) as anesthetic
Pressure transducer
Separator
Fluid catalyst regeneration process
Breast pads
Drawer organizer